Abstract |
The aim of this study was to test whether prolonged administration of granulocyte colony-stimulating factor ( G-CSF) would allow the collection by leukapheresis of PBHP in patients with SAA. For this purpose, nine SAA patients, 7 to 46 years old, six of whom were enrolled at diagnosis of their disease and three after previous immunosuppression had failed, were treated with antilymphocyte globulin (ALG) (day 1 to 5), cyclosporin A (5 mg/kg/d orally) (day 6 to 90) and G-CSF 5 micrograms/kg/d (day 6 to 90). A total of 40 leukaphereses were performed, (range 2 to 7 per patient), between days +10 and +168 from G-CSF treatment. White blood cell count at the time of harvest ranged from 1.2 to 18.1 x 10(9)/L. Results can be summarized as follows: the median number of cells collected per patient was 5.0 x 10(8)/kg (range 2.6 to 18.7), the median number of CD34+ cells was 1.8 x 10(6)/kg (range 0.27 to 3.8) and the median number of colony-forming units granulocyte-macrophage (CFU-GM) was 3.9 x 10(4)/kg (range 0 to 39). Twenty leukaphereses performed between days +33 and +77 of G-CSF treatment grew granulocyte macrophages and erythroid colonies in vitro. No colony growth was obtained from 20 leukaphereses performed before day +33 or after day +80. In six patients the total number of CFU-GM recovered were in the range described for autologous peripheral blood stem cell grafts. (2.6 to 39 x 10(4)/kg). In conclusion, this study suggests that circulating hematopoietic progenitors can be recovered after ALG priming and after at least 1 month of G-CSF treatment in a proportion of patients with SAA. Whether these cells will be suitable for autologous transplantation remains to be determined.
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Authors | A Bacigalupo, G Piaggio, M Podestá, M T Van Lint, M Valbonesi, G Lercari, P G Mori, M Pasino, E Franchini, L Rivabella |
Journal | Blood
(Blood)
Vol. 82
Issue 5
Pg. 1410-4
(Sep 01 1993)
ISSN: 0006-4971 [Print] United States |
PMID | 7689868
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- Antigens, Surface
- Antilymphocyte Serum
- Granulocyte Colony-Stimulating Factor
- Cyclosporine
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Topics |
- Adolescent
- Adult
- Anemia, Aplastic
(blood, drug therapy)
- Antigens, CD
(analysis)
- Antigens, Surface
(analysis, genetics)
- Antilymphocyte Serum
(therapeutic use)
- Child
- Child, Preschool
- Cyclosporine
(therapeutic use)
- Female
- Granulocyte Colony-Stimulating Factor
(administration & dosage)
- Hematopoietic Stem Cells
(cytology, drug effects)
- Humans
- Lymphocyte Depletion
- Male
- Middle Aged
- Phenotype
- T-Lymphocytes
(cytology)
- Time Factors
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