Aberrant expression of epidermal growth factor receptor in aural cholesteatoma.

Abstract

A monoclonal antibody recognizing an epitope of the external domain of the human epidermal growth factor (EGF) receptor was used in an alkaline phosphatase-antialkaline phosphatase (APAAP) technique to compare the distribution of this protein in normal human skin and aural cholesteatoma. EGF receptors appear to be highly expressed on the basal layer of the epidermis, in hair follicle apocrine sweat glands, and in the capillary system of normal skin. Cholesteatoma epithelium showed increased positive reactions in the suprabasal layers. A heterogeneity in the expression was found in different parts of the cholesteatoma. These results suggest the presence of an aberrant regulation and persistence of EGF receptors in cholesteatoma and confirm the hyperproliferative character of the cholesteatoma epithelium.

• Authors: J Bujía, A Holly, V Schilling, B Negri, P Pitzke, P Schulz
• Journal: The Laryngoscope (Laryngoscope) Vol. 103 Issue 3 Pg. 326-9 (Mar 1993) ISSN: 0023-852X [Print] United States
• PMID: 7680089 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ErbB Receptors
• Alkaline Phosphatase

Topics

• Alkaline Phosphatase
• Cell Membrane (metabolism, ultrastructure)
• Cholesteatoma (metabolism, pathology)
• Cytoplasmic Granules (metabolism, ultrastructure)
• Ear Diseases (metabolism, pathology)
• Epithelium (metabolism, pathology)
• ErbB Receptors (analysis, genetics)
• Gene Expression
• Humans
• Keratinocytes (metabolism, pathology)
• Skin (chemistry)
• Staining and Labeling