Association of sialyl-Lewis(a) and sialyl-Lewis(x) with MUC-1 apomucin ina pancreatic cancer cell line.

Abstract

We have shown previously that the mucins of the human pancreatic cancer cell line, SW1990, have both sialyl-Lewis(a) and sialyl-Lewis(x) carbohydrate ligands that are implicated in tumor cell metastasis. In the present study, we undertook to identify the protein core of these mucins. SW1990 mucins that carry sialyl-Lewis(a) and sialyl-Lewis(x) bound to the MUC1 peptide-specific mAb 139H2. Removal of most of the sialic acids from SW1990 mucins by neuraminidase greatly enhanced binding of two other MUC1 peptide specific antibodies, HMFG-2 and SM-3. After removal of sialic acids, most of the mucins rich in sialyl-Lewis(a) and sialyl-Lewis(x) oligosaccharides no longer bound to a DEAE-cellulose column at pH 8.0. These results indicate that at least part of the sialyl-Lewis(a) and sialyl-Lewis(x) in SW1990 cells is associated with the MUC1 polypeptide. Moreover, sialic acids play an important role in determining the net negative charge of sialyl-Lewis(a) and sialyl-Lewis(x) rich mucins and in obscuring MUC1 peptide regions.

• Authors: J J Ho, B Siddiki, Y S Kim
• Journal: Cancer research (Cancer Res) Vol. 55 Issue 16 Pg. 3659-63 (Aug 15 1995) ISSN: 0008-5472 [Print] United States
• PMID: 7627976 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Neoplasm
• Antigens, Tumor-Associated, Carbohydrate
• CA-19-9 Antigen
• Gangliosides
• Membrane Glycoproteins
• Mucin-1
• Mucins
• Peptides
• Sialyl Lewis X Antigen
• sialyl Lewis(x) ganglioside
• sialyl Le(a) ganglioside
• Neuraminidase

Topics

• Amino Acid Sequence
• Antibodies, Neoplasm (immunology)
• Antibody Specificity
• Antigens, Tumor-Associated, Carbohydrate (chemistry, metabolism)
• CA-19-9 Antigen
• Carcinoma, Ductal, Breast (immunology, metabolism)
• Epitope Mapping
• Gangliosides (chemistry, metabolism)
• Humans
• In Vitro Techniques
• Membrane Glycoproteins (chemistry, metabolism)
• Molecular Sequence Data
• Mucin-1
• Mucins (chemistry, metabolism)
• Neuraminidase (pharmacology)
• Pancreatic Neoplasms (immunology, metabolism)
• Peptides (chemistry, immunology)
• Sialyl Lewis X Antigen
• Tumor Cells, Cultured