The levels of collagenase, tissue inhibitor of metalloproteinases-1 (TIMP-1), collagenase approximately TIMP-1 complexes and glycosaminoglycan (GAG) in sequential samples of synovial fluid aspirated from patients with osteoarthritis.

Abstract

OBJECTIVE:

Collagen turnover in connective tissues is thought to be controlled by the balance between the levels of interstitial collagenase and tissue inhibitor of metalloproteinases (TIMP-1). The aim of this study was to measure the level of total collagenase (MMP-1), TIMP-1, collagenase approximately TIMP-1 complex and glycosaminoglycan (GAG) in sequential samples of osteoarthritic knee synovial fluid from well documented patients to determine if these parameters changed with time and correlated with clinical indices.

METHODS:

Twenty-one patients were recruited and randomly allocated to receive tiaprofenic acid, indomethacin or naproxen. Total collagenase, TIMP-1, collagenase approximately TIMP-1 complex and GAG were measured in 80 osteoarthritic synovial fluids taken over a period of six months.

RESULTS:

The majority of fluids contained a molar excess of TIMP-1 over collagenase, although in seven fluids collagenase was present in excess; six of these samples were from a single patient. GAG levels were relatively unchanged over the six months studied.

CONCLUSION:

The levels of collagenase and TIMP-1 varied between patients and over time in individual patients. No collagenase approximately TIMP-1 complex was found in any fluid. There was no significant difference in the median levels of collagenase, TIMP-1 or GAG in the different treatment groups. High levels of collagenase were found in one patient with a crystal related disease. These immunoassays give valuable information on the levels of collagenase and TIMP-1 in individual patients with time and may help to determine the mechanisms controlling the turnover of cartilage collagen in different arthritides.

• Authors: T E Cawston, V Curry, S Ramsey, I M Clark, V A Kyle, A Adebajo, B Silverman, T Daymond, B L Hazleman
• Journal: Clinical and experimental rheumatology (Clin Exp Rheumatol) 1995 Jul-Aug Vol. 13 Issue 4 Pg. 431-7 ISSN: 0392-856X [Print] Italy
• PMID: 7586773 (Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Glycoproteins
• Glycosaminoglycans
• Matrix Metalloproteinase Inhibitors
• Propionates
• Tissue Inhibitor of Metalloproteinases
• tiaprofenic acid
• Naproxen
• Collagenases
• Indomethacin

Topics

• Adult
• Aged
• Anti-Inflammatory Agents, Non-Steroidal (therapeutic use)
• Collagenases (metabolism)
• Enzyme-Linked Immunosorbent Assay
• Female
• Glycoproteins (metabolism)
• Glycosaminoglycans (metabolism)
• Humans
• Indomethacin (therapeutic use)
• Knee Joint
• Male
• Matrix Metalloproteinase Inhibitors
• Middle Aged
• Naproxen (therapeutic use)
• Osteoarthritis (drug therapy, metabolism)
• Propionates (therapeutic use)
• Suction
• Synovial Fluid (metabolism)
• Tissue Inhibitor of Metalloproteinases