Pharmacological effects of recombinant human granulocyte colony-stimulating factor modified by polyethylene glycol on anticancer drug-induced neutropenia in mice.

Abstract

1. To clarify the pharmacological effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) conjugated to polyethylene glycol (PEG), its effects on the number of circulating neutrophils in mice made neutropenic by cyclophosphamide (CPA) or 5-fluorouracil (5-FU) were compared with rhG-CSF lacking PEG. 2. In normal mice, PEG-conjugated rhG-CSF (PEG-rhG-CSF, 10 micrograms protein/kg) induced an increase in neutrophils which lasted for 72 h after injection whereas the effect of rhG-CSF (10 micrograms protein/kg) disappeared by 24 h after injection. 3. In CPA or 5-FU-induced neutropenic mice, PEG-rhG-CSF inhibited neutropenia or accelerated recovery from neutropenia and its potency was higher than that of rhG-CSF. 4. These results indicate that PEG-rhG-CSF has a longer duration of action than rhG-CSF and is more effective in the recovery from neutropenia.

• Authors: M Ishikawa, Y Okada, R Satake-Ishikawa, M Kakitani, M Kawagishi, S Matsuki, M Kusaka, K Asano
• Journal: General pharmacology (Gen Pharmacol) Vol. 25 Issue 3 Pg. 533-7 (May 1994) ISSN: 0306-3623 [Print] England
• PMID: 7523237 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Recombinant Proteins
• Granulocyte Colony-Stimulating Factor
• Polyethylene Glycols
• Cyclophosphamide
• Fluorouracil

Topics

• Animals
• Cyclophosphamide (adverse effects, antagonists & inhibitors)
• Drug Evaluation, Preclinical
• Female
• Fluorouracil (adverse effects)
• Granulocyte Colony-Stimulating Factor (antagonists & inhibitors, chemistry, pharmacology)
• Humans
• Male
• Mice
• Mice, Inbred ICR
• Mice, Inbred Strains
• Neutropenia (chemically induced, drug therapy)
• Polyethylene Glycols (chemistry, pharmacology)
• Recombinant Proteins (antagonists & inhibitors, chemistry, pharmacology)