Influence of various forms of dialyzable leukocyte extracts on rat adjuvant arthritis.

Abstract

Adjuvant-induced arthritis in rats is a chronic inflammatory disease, widely used as an animal model for rheumatoid arthritis. In our study the effect of various fractions of dialyzable leukocyte extract (DLE): DLE I-molecular weight below 10 kDa (commercial preparation), DLE II-molecular weight below 5 kDa (suppressor fraction), DLE III-molecular weight 5-10 kDa on rat adjuvant-induced arthritis was studied. The adjuvant arthritic (AA) rats were treated with DLE fractions i.p. in solutions containing an active substance isolated from 12.5 x 10(6) and 6.25 x 10(6) leukocytes from day 1 (adjuvant injected) through day 18, every second day (total 9 times). Various markers of inflammation, immune function and joint destruction were evaluated: hindpaw volume, serum hyaluronic acid, serum albumin and biopterin in urine. All these markers showed a significant improvement after using fraction DLE II in comparison with AA controls. Fractions DLE I and DLE III influenced only some markers of inflammation and immune function. Our results demonstrated a therapeutical effect of fraction DLE II on rat adjuvant-induced arthritis.

• Authors: M Stanciková, J Rovenský, J Pekárek, E Orviský, S Blazícková, K Cech
• Journal: Archivum immunologiae et therapiae experimentalis (Arch Immunol Ther Exp (Warsz)) Vol. 42 Issue 4 Pg. 295-9 ( 1994) ISSN: 0004-069X [Print] Switzerland
• PMID: 7487370 (Publication Type: Journal Article)

Chemical References

• Cell Extracts
• Serum Albumin
• Biopterin
• Hyaluronic Acid

Topics

• Animals
• Arthritis, Experimental (metabolism, pathology, therapy)
• Arthritis, Rheumatoid
• Biopterin (urine)
• Cell Extracts (isolation & purification, therapeutic use)
• Dialysis
• Disease Models, Animal
• Hyaluronic Acid (blood)
• Leukocytes (chemistry)
• Male
• Rats
• Rats, Inbred Lew
• Serum Albumin (analysis)