Abstract |
The intracellular localization of calcium during contraction of striated flagellar roots of the green alga Tetraselmis subcordiformis was investigated using calcium pyroantimonate and calcium oxalate cytochemistry and energy-dispersive X-ray microanalysis. Vesicles (0.25 - 0.50 microns diameter) located in the anterior-most region of the cell are calcium-sequestering organelles. These vesicles exhibit a consistent and reproducible localization of electron-opaque pyroantimonate and oxalate salts of calcium. Striated contractile flagellar roots (CFR) show a periodic pattern of calcium antimonate (Ca-Sb) deposits following ' calcium shock' stimulation of contraction of this organelle. When CFRs are in a fully extended state they do not show Ca-Sb deposits. Deposits of Ca-Sb precipitate are present throughout the cytosol when CFRs are stimulated to contract. The implications of these findings with respect to the regulation of intracellular levels of 'free' Ca2+ and to the mechanism of CFR contraction are discussed.
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Authors | J L Salisbury |
Journal | Journal of cell science
(J Cell Sci)
Vol. 58
Pg. 433-43
(Dec 1982)
ISSN: 0021-9533 [Print] England |
PMID | 7183697
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Calcium
(analysis)
- Chlorophyta
(analysis, ultrastructure)
- Electron Probe Microanalysis
- Flagella
(analysis, physiology, ultrastructure)
- Microscopy, Electron
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