The form factor of alpha-naphthyl acetate esterase-positive cells in non-Hodgkin's lymphomas and reactive lymph nodes.

Abstract

The shape of alpha-naphthyl acetate esterase (ANAE)-positive cells (other than T lymphocytes) has been measured in 40 lymph nodes. The specimens comprised 15 high-grade lymphomas, 15 low-grade lymphomas and 10 reactive lymph nodes. The parameter used for the measurement of shape was form factor (FF), which is readily calculated by the Reichert-Jung (Kontron) MOP-AMO3 user-controlled image analyzer. Perfectly round cells have an FF value of 1.0, whereas the FF of irregularly-shaped cells diverges from unity. It has been demonstrated that the ANAE-positive cells in high-grade lymphomas have mean values for FF of 0.8-0.9, whereas in low-grade lymphomas and reactive nodes the mean value is 0.4-0.5. Thus, high-grade lymphomas contain many more rounded ANAE-positive macrophage type cells than do low-grade lymphomas and reactive nodes. In the latter two types of specimen there is an excess of branching and spindle-shaped ANAE-positive cells.

• Authors: J Crocker, E L Jones, R C Curran
• Journal: Journal of clinical pathology (J Clin Pathol) Vol. 36 Issue 3 Pg. 303-6 (Mar 1983) ISSN: 0021-9746 [Print] England
• PMID: 6338056 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Naphthol AS D Esterase
• Carboxylic Ester Hydrolases

Topics

• Carboxylic Ester Hydrolases (analysis)
• Histological Techniques
• Humans
• Hyperplasia (enzymology)
• Lymph Nodes (enzymology, pathology)
• Lymphoma (enzymology, pathology)
• Mathematics
• Naphthol AS D Esterase (analysis)