Uridine kinase molecular species and uridine uptake in some variants of rat hepatomas.

Abstract

In a family of clonal lines derived from the Reuber H 35 rat hepatoma, four electrophoretically distinct molecular forms of uridine kinase (UK I, II, III, and IV) have been characterized. They are the same as those found in foetal rat liver. Different UK profiles occur in these cell lines, and no strict correlation could be established between the state of differentiation of the cells and the form of UK expressed. A clone of somatic hybrid cells between line p4 (form 1 only) and Fu5-5 (forms II, III, and IV) that does not express form I indicates that p4 cells may lack a factor controlling the polymerization of form I. This variety of clonal cell lines was used to study the uptake and phosphorylation of labeled uridine. The results suggest a relationship between the UK form present and the rate uridine phosphorylation by the intact cells.

• Authors: M C Fulchignoni-Lataud, J M Roux
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 118 Issue 1 Pg. 34-8 (Jan 1984) ISSN: 0021-9541 [Print] United States
• PMID: 6317703 (Publication Type: Journal Article)

Chemical References

• Phosphotransferases
• Uridine Kinase
• Uridine

Topics

• Animals
• Cell Line
• Liver Neoplasms, Experimental (metabolism)
• Phosphorylation
• Phosphotransferases (analysis)
• Rats
• Uridine (metabolism)
• Uridine Kinase (analysis)