Reye's syndrome simulacra in liver of mice after treatment with chemical agents and encephalomyocarditis virus.

Abstract

In children with Reye's syndrome, liver specimens exhibit the following characteristics: mitochondrial dysfiguration, fatty infiltration, decreased activity of carbamyl phosphate synthetase and of ornithine transcarbamylase, histochemically reduced activity of succinic dehydrogenase and cytochrome oxidase, and depletion of glycogen. We intended to create an animal model for Reye's syndrome by treating mice with encephalomyocarditis virus, and/or salicylate, fructose, Atlox, butylated hydroxytoluene, pentachlorophenol, and an equal mixture of butylated hydroxytoluene and monosodium stearate. Liver specimens were then examined for the listed characteristics as well as for the activity of argininosuccinic lyase, arginase, phosphorylase, and glucose-6-phosphatase. Results of interest in regard to the experimental intention were obtained in livers of mice treated with virus and Atlox (A) or virus and butylated hydroxytoluene (B). In these specimens, we found a significant reduction (p less than 0.05)--except for ornithine transcarbamylase (A)--to the following levels (in percentage of normal mean): carbamyl phosphate synthetase (A, 79 per cent; B, 57 per cent); ornithine transcarbamylase (A, 91 per cent; B, 75 per cent); glycogen (A, 26 per cent; B, 37 per cent). Simultaneous morphologic analysis of these liver specimens indicated mitochondrial dysfiguration, absence of dense granules, fatty infiltration, and normal activity of succinic dehydrogenase and cytochrome oxidase. The induction of Reye's syndrome-like features in mouse liver may be useful for the study of disease mechanisms and therapy.

• Authors: G Hug, J Bosken, K Bove, C C Linnemann Jr, L McAdams
• Journal: Laboratory investigation; a journal of technical methods and pathology (Lab Invest) Vol. 45 Issue 1 Pg. 89-109 (Jul 1981) ISSN: 0023-6837 [Print] United States
• PMID: 6265702 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Salicylates
• Surface-Active Agents
• Fructose
• Pentachlorophenol

Topics

• Animals
• Encephalomyocarditis virus
• Enterovirus Infections (etiology)
• Fructose (pharmacology)
• Liver (metabolism, microbiology, pathology, ultrastructure)
• Mice
• Pentachlorophenol (pharmacology)
• Reye Syndrome (diagnosis)
• Salicylates (pharmacology)
• Surface-Active Agents (pharmacology)