Changes in the expression of two Epstein-Barr virus-associated antigens, EBNA and RANA, during the cell cycle of transformed human B lymphoblasts.

Abstract

Two methods of cell sychronization, density-dependent arrest and double thymidine block, were used to assign two Epstein-Barr virus-associated antigens to different parts of the growth cycle of the human B lymphblastoid cell lines, WI-L2 and Raji. The Epstein-Barr nuclear antigen (EBNA), as detected by anti-complement immunofluorescence, was maximally expressed during early S phase, decreased during the G2 and M phases, and was absent in early G1. In contrast, the rheumatoid arthritis nuclear antigen (RANA), as detected by anti-immunoglobulin immunofluorescence with a prototype serum form a patient with rheumatoid arthritis, was maximally expressed during early G1 phase, progressively decreased during S and early G2, and reappeared during late G2/M.

• Authors: S F Slovin, J H Vaughan, D A Carson
• Journal: International journal of cancer (Int J Cancer) Vol. 26 Issue 1 Pg. 9-12 (Jul 15 1980) ISSN: 0020-7136 [Print] United States
• PMID: 6263806 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Viral
• Autoantigens
• Epstein-Barr Virus Nuclear Antigens
• rheumatoid arthritis nuclear antigen

Topics

• Antigens, Viral (analysis)
• Arthritis, Rheumatoid (immunology)
• Autoantigens
• B-Lymphocytes (immunology, pathology)
• Cell Cycle
• Cell Line
• Cell Nucleus (immunology)
• Epstein-Barr Virus Nuclear Antigens
• Herpesvirus 4, Human (immunology)
• Humans