T-cell subsets regulating anti-acetylcholine-receptor-antibody formation in myasthenia gravis and characterization of suppressor T-cell factors involved.

Abstract

The in vitro generation of anti-acetylcholine-receptor (AChR)-antibody-forming cells from B cells of myasthenia gravis (MG) patients occurred only in the presence of OKT4+ cells. Anti-AChR-antibody formation by lymphocytes from MG patients was suppressed by normal T cells, and the requirement of both OKT4+ and OKT8+ cells was suggested for this suppression. Culture supernatant from normal T cells stimulated with excess doses of AChR antigen, but not T cells from patients, suppressed anti-AChR-antibody formation by lymphocytes from patients. The fraction of the culture supernatant with molecular weight of 45,000 to 67,000 seemed to contain an AChR-antigen-specific and antigen-binding suppressor factor with some allogeneic barrier, while the fraction with a molecular weight of 10,000 to 28,000 contained the antigen-nonspecific factor.

• Authors: N Shinomiya, J Yata, T Sasazuki
• Journal: Clinical immunology and immunopathology (Clin Immunol Immunopathol) Vol. 33 Issue 2 Pg. 182-90 (Nov 1984) ISSN: 0090-1229 [Print] United States
• PMID: 6207969 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Epitopes
• HLA Antigens
• Receptors, Cholinergic

Topics

• Antibody Formation
• Antibody-Producing Cells
• B-Lymphocytes (immunology)
• Binding Sites
• Cells, Cultured
• Epitopes
• HLA Antigens (immunology)
• Humans
• Myasthenia Gravis (immunology)
• Receptors, Cholinergic (immunology)
• T-Lymphocytes (classification, immunology)
• T-Lymphocytes, Regulatory (immunology)