The use of non-deparaffinized tissue sections for staining leprosy bacilli.

Abstract

Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-carbol pararosanilin stain or methenamine silver stain for demonstrating mycobacteria.

• Authors: K Harada
• Journal: International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association (Int J Lepr Other Mycobact Dis) 1976 Jul-Sep Vol. 44 Issue 3 Pg. 366-8 ISSN: 0148-916X [Print] United States
• PMID: 61950 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Rosaniline Dyes
• Paraffin

Topics

• Humans
• Leprosy (microbiology)
• Mycobacterium leprae (isolation & purification)
• Paraffin
• Rosaniline Dyes
• Staining and Labeling