Multiple endorphins in neuronal hybrid cell lines.

Abstract

Neuroblastoma x glioma hybrid cells (NG 108CC15) and tumors derived thereof were examined for dynorphin- and alpha-neoendorphin-like material. The techniques employed for analyses of opiate-like material were the isolated mouse vas deferens bioassay and gel chromatography and high pressure liquid chromatography in combination with radioimmunoassays. Dynorphin- and alpha-neoendorphin-like material was detected in both the hybrid cells and the corresponding tumors. Immunoreactive dynorphin and alpha-neoendorphin was also in NCB 20 hybrid cells and in tumors thereof assayed. In all samples investigated, the amounts of alpha-neoendorphin-like material was higher than that of dynorphin-like material. The results revealed considerable variability in the amount of dynorphin- and alpha-neoendorphin-activity between particular samples, suggesting the need for studies into the responsible mechanisms.

• Authors: R Schulz, C Gramsch, M Wüster
• Journal: Neuropeptides (Neuropeptides) Vol. 3 Issue 4 Pg. 271-83 (Apr 1983) ISSN: 0143-4179 [Print] Netherlands
• PMID: 6135175 (Publication Type: Journal Article)

Chemical References

• Endorphins
• alpha-Endorphin
• Dynorphins

Topics

• Animals
• Biological Assay
• Cell Line
• Chromatography, Gel
• Chromatography, High Pressure Liquid
• Dynorphins
• Endorphins (analysis, pharmacology)
• Glioma (analysis)
• Hybrid Cells (analysis)
• Male
• Mice
• Neuroblastoma (analysis)
• Radioimmunoassay
• Rats
• Vas Deferens (drug effects)
• alpha-Endorphin