Detection of induced tumour-resistance to cyclophosphamide by the in vitro short term test.

Abstract

Treatment of the solid Walker carcinosarcoma of the rat for a period of one year with cyclophosphamide (40 mg/kg at each passage; tumours were transplanted weekly) resulted in the formation of a cyclophosphamide-resistant tumour cell line. Without further treatment, animals injected with non-treated or cyclophosphamide pre-treated cells survived for 10 days on an average. After therapy with cyclophosphamide (2 x 38 mg/kg), rats with non-treated tumour cells survived 22 days whereas those with pre-treated tumours survived only for 17 days. Tumour cells which were shown to be sensitive in vivo also exhibited a larger reduction in 3H-thymidine and 3H-uridine incorporation in the in vitro short term test after incubation with urine from cyclophosphamide-treated rats or with 4-hydroperoxy-cyclophosphamide. The resistance to cyclophosphamide which was detected in animal experiments with Walker carcinosarcoma can therefore also be observed using the in vitro short term test.

• Authors: M Volm, J Mattern, K Wayss
• Journal: Archiv fur Gynakologie (Arch Gynakol) Vol. 223 Issue 4 Pg. 249-57 (Nov 29 1977) ISSN: 0003-9128 [Print] Germany
• PMID: 579579 (Publication Type: Journal Article)

Chemical References

• Cyclophosphamide

Topics

• Animals
• Biotransformation
• Carcinoma 256, Walker (drug therapy, metabolism)
• Cell Survival (drug effects)
• Cyclophosphamide (adverse effects, metabolism, therapeutic use)
• Drug Resistance
• In Vitro Techniques
• Male
• Microsomes, Liver (metabolism)
• Rats
• Time Factors