Abstract |
1. When Escherichia coli leu(-) was incubated at 35 degrees in a medium based on minimal medium, but with the omission of phosphate ions, or glucose, or NH(4) (+) ions and leucine, intracellular protein was degraded at a rate of about 5%/hr. in each case. If Mg(2+) ions were omitted, however, the rate of degradation was 2.9%/hr. 2. Under certain conditions of incubation, protein degradation was inhibited. The inhibitor was neither NH(4) (+) ions nor amino acids, and its properties were not those of a protein, but it might be an unstable species of RNA. 3. Although a large part of the cell protein was degraded at about 5%/hr. during starvation of NH(4) (+) ions and leucine, some proteins were lost at more rapid rates, whereas others were lost at lower rates or not at all. 4. In particular, beta-galactosidase activity was lost at about 8%/hr. during starvation of NH(4) (+) ions and leucine, whereas d-serine-deaminase and alkaline-phosphatase activities were stable. During starvation of Mg(2+) ions, all three enzyme activities were stable.
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Authors | N S Willetts |
Journal | The Biochemical journal
(Biochem J)
Vol. 103
Issue 2
Pg. 453-61
(May 1967)
ISSN: 0264-6021 [Print] England |
PMID | 5340366
(Publication Type: Journal Article)
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Chemical References |
- Amino Acids
- Bacterial Proteins
- Carbon Isotopes
- Nucleosides
- Phosphates
- Quaternary Ammonium Compounds
- RNA
- Chloramphenicol
- Alkaline Phosphatase
- Galactosidases
- Hydro-Lyases
- Leucine
- Valine
- Magnesium
- Glucose
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Topics |
- Alkaline Phosphatase
(metabolism)
- Amino Acids
(analysis)
- Bacterial Proteins
(metabolism)
- Bacteriological Techniques
- Bacteriolysis
- Carbon Isotopes
- Chloramphenicol
(pharmacology)
- Enzyme Induction
- Escherichia coli
(growth & development, metabolism)
- Galactosidases
(metabolism)
- Glucose
(metabolism)
- Hydro-Lyases
(metabolism)
- Kinetics
- Leucine
(metabolism)
- Magnesium
(metabolism)
- Nucleosides
- Phosphates
(metabolism)
- Quaternary Ammonium Compounds
(pharmacology)
- RNA
(pharmacology)
- Ultrasonics
- Valine
(analysis)
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