Formalin-inactivated Venezuelan equine encephalomyelitis (Trinidad strain) vaccine produced in rolling-bottle cultures of chicken embryo cells.

Abstract

Formalin-inactivated Venezuelan equine encephalomyelitis vaccine was prepared from virus grown in rolling-bottle cultures of chicken embryo cells. Trinidad strain virus was propagated in these cultures with a maintenance medium consisting of serum-free medium 199 containing 0.25% human serum albumin (USP) and antibiotics. Manipulation of multiplicity of inoculum (0.06 to 0.00006) and maintenance medium volume (100 to 300 ml) resulted in high-titered virus yields and only moderate cell destruction when fluids from infected cultures were harvested at 18 to 24 hr. The virus was inactivated at 37 C by 0.05% Formalin within 8 to 10 hr and with 0.1% Formalin within 6 to 8 hr. Single dose, antigen extinction tests in mice performed with 30 small-scale vaccine lots showed excellent potency at either Formalin concentration with inactivation periods ranging from 24 to 96 hr.

• Authors: F E Cole Jr, S W May, D M Robinson
• Journal: Applied microbiology (Appl Microbiol) Vol. 25 Issue 2 Pg. 262-5 (Feb 1973) ISSN: 0003-6919 [Print] United States
• PMID: 4694345 (Publication Type: Journal Article)

Chemical References

• Culture Media
• Viral Vaccines
• Formaldehyde

Topics

• Animals
• Chick Embryo
• Culture Media
• Culture Techniques
• Encephalitis Virus, Venezuelan Equine (drug effects, growth & development, immunology)
• Formaldehyde (pharmacology)
• Mice
• Viral Vaccines (pharmacology, standards)
• Virus Cultivation