Abstract | Introduction: Methods: We analyzed the cortical, hippocampal, ventricular, and atrial protein lysates from APP/PS1 mice and their respective controls for inflammasome signaling activation. Additionally, we analyzed serum-derived EV for size, concentration, and content of inflammasome proteins as well as the EV marker CD63. Finally, we performed conditioned media experiments of EV from AD patients and healthy age-matched controls delivered to cardiovascular cells in culture to assess EV-induced inflammation. Results: We show a significant increase in Pyrin, NLRP1, caspase-1, and ASC in the brain cortex whereas caspase-8, ASC, and IL-1β were significantly elevated in the heart ventricles of AD mice when compared to controls. We did not find significant differences in the size or concentration of EV between groups, but there was a significant increase of caspase-1 and IL-1β in EV from AD mice compared to controls. In addition, conditioned media experiments of serum-derived EV from AD patients and age-matched controls delivered to cardiovascular cells in culture resulted in inflammasome activation, and significant increases in TNF-α and IL-2. Conclusion:
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Authors | Brianna Cyr, Erika D L R M Cabrera Ranaldi, Roey Hadad, W Dalton Dietrich, Robert W Keane, Juan Pablo de Rivero Vaccari |
Journal | Frontiers in molecular neuroscience
(Front Mol Neurosci)
Vol. 17
Pg. 1369781
( 2024)
ISSN: 1662-5099 [Print] Switzerland |
PMID | 38660388
(Publication Type: Journal Article)
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Copyright | Copyright © 2024 Cyr, Cabrera Ranaldi, Hadad, Dietrich, Keane and de Rivero Vaccari. |