[MiR-26-3p regulates proliferation, migration, invasion and apoptosis of glioma cells by targeting CREB1].

Abstract

OBJECTIVE:

To investigate the regulatory role of miR-26b-3p in proliferation, migration and invasion of glioma.

METHODS:

The expressions of miR-26b-3p and cAMP-responsive element binding protein 1 (CREB1) in gliomas of different pathological grades were detected with RT-qPCR and Western blotting. Bioinformatic methods were used to analyze the target sequence of miRNA-26b-3p binding to CREB1, and dual luciferase gene reporter experiment was performed to explore the mechanism for targeted regulation of CREB1 by miR-26b-3p. Glioma U251 cells were treated with miR-26b-3p mimic or inhibitor, and the changes in CREB1 expression and cell proliferation, migration, invasion and apoptosis were determined with Western blotting, CCK-8 assay, wound healing assay, Transwell assay, and flow cytometry.

RESULTS:

The expression of miR-26b-3p decreased while CREB1 expression increased significantly as the pathological grade of gliomas increased (P < 0.05). Dual luciferase gene reporter experiment confirmed that CREB1 was a downstream target of miR-26b-3p. Inhibition of miR-26b-3p significantly upregulated the expression of CERB1, suppressed apoptosis and promoted proliferation and invasion of glioma cells, and overexpression of miR-26b-3p produced the opposite effects (P < 0.05).

CONCLUSION:

MiR-26b-3p regulates CREB1 expression to modulate apoptosis, proliferation, migration and invasion of glioma cells, thereby participating in tumorigenesis and progression of glioma.

• Authors: Q Huang, J Zhou, Z Wang, K Yang, Z Chen
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 44 Issue 3 Pg. 578-584 (Mar 20 2024) ISSN: 1673-4254 [Print] China
• PMID: 38597450 (Publication Type: English Abstract, Journal Article)

Chemical References

• CREB1 protein, human
• Cyclic AMP Response Element-Binding Protein
• Luciferases
• MicroRNAs
• MIRN26 microRNA, human

Topics

• Humans
• Apoptosis (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Cyclic AMP Response Element-Binding Protein (metabolism)
• Gene Expression Regulation, Neoplastic
• Glioma (genetics, pathology)
• Luciferases (genetics)
• MicroRNAs (metabolism)