Single-cell RNA-sequencing of virus-specific cellular immune responses in chronic hepatitis B patients.

Abstract

Chronic hepatitis B (CHB) is a major global health challenge. CHB can be controlled by antivirals but a therapeutic cure is lacking. CHB is characterized by limited HBV-specific T cell reactivity and functionality and expression of inhibitory receptors. The mechanisms driving these T cell phenotypes are only partially understood. Here, we created a single-cell RNA-sequencing dataset of HBV immune responses in patients to contribute to a better understanding of the dysregulated immunity. Blood samples of a well-defined cohort of 21 CHB and 10 healthy controls, including a subset of 5 matched liver biopsies, were collected. scRNA-seq data of total immune cells (55,825) plus sorted HBV-specific (1,963), non-naive (32,773) and PD1+ T cells (96,631) was generated using the 10X Genomics platform (186,123 cells) or the full-length Smart-seq2 protocol (1,069 cells). The shared transcript count matrices of single-cells serve as a valuable resource describing transcriptional changes underlying dysfunctional HBV-related T cell responses in blood and liver tissue and offers the opportunity to identify targets or biomarkers for HBV-related immune exhaustion.

• Authors: Klas Hatje, Tony Kam-Thong, Nicolas Giroud, Antonio Saviano, Pauline Simo-Noumbissie, Nadine Kumpesa, Tobias Nilsson, François Habersetzer, Thomas F Baumert, Nadege Pelletier, Marianne Forkel
• Journal: Scientific data (Sci Data) Vol. 11 Issue 1 Pg. 355 (Apr 08 2024) ISSN: 2052-4463 [Electronic] England
• PMID: 38589415 (Publication Type: Dataset, Journal Article)
• Copyright: © 2024. The Author(s).

Chemical References

• RNA

Topics

• Humans
• Hepatitis B virus
• Hepatitis B, Chronic (genetics, immunology)
• Immunity, Cellular
• RNA
• Single-Cell Analysis
• Sequence Analysis, RNA
• T-Lymphocytes (immunology)
• Liver (virology)