Resveratrol ameliorates glioblastoma inflammatory response by reducing NLRP3 inflammasome activation through inhibition of the JAK2/STAT3 pathway.

Abstract

OBJECTIVES:

The aim of this study was to investigate the anti-tumor effect of resveratrol (RSV) on glioblastoma (GBM) and its specific mechanism in improving the inflammatory response of the tumor microenvironment. The tumor microenvironment of GBM is highly neuroinflammatory, inducing tumor immunosuppression. Therefore, ameliorating the inflammatory response is an important focus for anti-tumor research.

METHODS:

The anti-tumor effect of RSV on GBM was demonstrated through in vitro cellular assays, including CCK-8, EdU, PI staining, Transwell, wound healing assay, and flow cytometry. Potential mechanisms of RSV's anti-GBM effects were identified through network pharmacological analysis. In addition, the relationship of RSV with the JAK2/STAT3 signaling pathway and the inflammasome NLRP3 was verified using Western blot.

RESULTS:

RSV significantly inhibited cell viability in GBM cell lines LN-229 and U87-MG. Furthermore, it inhibited the proliferation and invasive migration ability of GBM cells, while promoting apoptosis. Network pharmacological analysis revealed a close association between the anti-GBM effects of RSV and the JAK/STAT signaling pathway, as well as inflammatory responses. Western blot analysis confirmed that RSV inhibited the over-activation of the inflammasome NLRP3 through the JAK2/STAT3 signaling pathway. Partial reversal of RSV's inhibition of inflammasome NLRP3 was observed with the addition of the JAK/STAT agonist RO8191.

CONCLUSIONS:

In vitro, RSV can exert anti-tumor effects on GBM and improve the inflammatory response in the GBM microenvironment by inhibiting the activation of the JAK2/STAT3 signaling pathway. These findings provide new insights into potential therapeutic targets for GBM.

• Authors: Chao Zhang, Qian Peng, Yuhang Tang, Chengcheng Wang, Shuai Wang, Dong Yu, Shiqiang Hou, Yu Wang, Lanlan Zhang, Ning Lin
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 150 Issue 3 Pg. 168 (Mar 28 2024) ISSN: 1432-1335 [Electronic] Germany
• PMID: 38546908 (Publication Type: Journal Article)
• Copyright: © 2024. The Author(s).

Chemical References

• Resveratrol
• Inflammasomes
• NLR Family, Pyrin Domain-Containing 3 Protein
• STAT3 Transcription Factor
• Janus Kinase 2
• JAK2 protein, human
• STAT3 protein, human

Topics

• Humans
• Glioblastoma (pathology)
• Resveratrol (pharmacology, therapeutic use)
• Inflammasomes (metabolism)
• NLR Family, Pyrin Domain-Containing 3 Protein (metabolism)
• Brain Neoplasms (pathology)
• STAT3 Transcription Factor (metabolism)
• Cell Line, Tumor
• Janus Kinase 2 (metabolism)
• Tumor Microenvironment