The development of shape-memory
hemostatic agents is crucial for the treatment of deep incompressible
bleeding tissue. However, there are few reports on
biomaterials that can monitor
bacterial infection at the
wound site in real time following hemostasis and effectively promote repair. In this study, we propose a multifunctional QCSG/FLZ
cryogel composed of
glycidyl methacrylate-functionalized quaternary
chitosan (QCSG),
fluorescein isothiocyanate (
FITC), and a
lysozyme (LYZ)-modified zeolitic imidazolate framework (ZIF-8) for incompressible
bleeding tissue hemostasis and
wound repair. QCSG/FLZ
cryogels possess interconnected microporous structure and enhanced mechanical properties, allowing them to be molded into different shapes for effective hemostasis in deep incompressible
wounds. Furthermore, the fluorescence quench signal of QCSG/FLZ
cryogels enables timely monitoring of
bacterial infection when
wound triggers
infection. Meanwhile, the acidic microenvironment of
bacterial infection induces structural lysis of ZIF-8, releasing LYZ and Zn2+, which effectively kill bacteria and accelerate
wound repair. In conclusion, our study not only provides potential application of QCSG/FLZ
cryogels for hemostasis in deep incompressible
wounds but promisingly promotes the development of a tissue repair technique.