Acoustic Activation Imaging With Intravenous Perfluoropropane Nanodroplets Results in Selective Bioactivation of the Risk Area.

Abstract

BACKGROUND:

Acoustically activatable perfluoropropane droplets (PD) can be formulated from commercially available microbubble preparations. Diagnostic transthoracic ultrasound frequencies have resulted in acoustic activation (AA) predominately within myocardial infarct zones (IZ).

OBJECTIVE:

We hypothesized that the AA area following acute coronary ischemia/reperfusion (I/R) would selectively enhance the developing scar zone, and target bioeffects specifically to this region.

METHODS:

We administered intravenous PD in 36 rats and 20 pigs at various stages of myocardial scar formation (30 minutes, 1 day, and 7 days post I/R) to determine what effect infarct age had on the AA within the IZ. This was correlated with histology, myeloperoxidase activity, and tissue nitrite activity.

RESULTS:

The degree of AA within the IZ in rats was not associated with collagen content, neutrophil infiltration, or infarct age. AA within 24 hours of I/R was associated with increased nitric oxide utilization selectively within the IZ (P < .05 compared with remote zone). The spatial extent of AA in pigs correlated with infarct size only when performed before sacrifice at 7 days (r = .74, P < .01).

CONCLUSIONS:

Acoustic activation of intravenous PD enhances the developing scar zone following I/R, and results in selective tissue nitric oxide utilization.

• Authors: Shouqiang Li, Cheng Chen, John Lof, Elizabeth A Stolze, Jiri Sklenar, Xucai Chen, John J Pacella, Flordeliza S Villanueva, Terry O Matsunaga, E Carr Everbach, Stanley J Radio, Sherry N Westphal, Sruti Shiva, Feng Xie, Xiaoping Leng, Thomas R Porter
• Journal: Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine (J Ultrasound Med) Vol. 43 Issue 6 Pg. 1063-1080 (Jun 2024) ISSN: 1550-9613 [Electronic] England
• PMID: 38440926 (Publication Type: Journal Article)
• Copyright: © 2024 American Institute of Ultrasound in Medicine.

Chemical References

• Fluorocarbons
• perflutren
• Contrast Media

Topics

• Animals
• Fluorocarbons (pharmacokinetics)
• Swine
• Rats
• Myocardial Infarction (diagnostic imaging)
• Male
• Contrast Media (pharmacokinetics)
• Nanoparticles
• Rats, Sprague-Dawley
• Myocardium (metabolism)
• Disease Models, Animal
• Myocardial Reperfusion Injury (diagnostic imaging)
• Microbubbles
• Female
• Ultrasonography (methods)