Astroglial calcium signaling and homeostasis in tuberous sclerosis complex.

Abstract

Tuberous Sclerosis Complex (TSC) is a multisystem genetic disorder characterized by the development of benign tumors in various organs, including the brain, and is often accompanied by epilepsy, neurodevelopmental comorbidities including intellectual disability and autism. A key hallmark of TSC is the hyperactivation of the mechanistic target of rapamycin (mTOR) signaling pathway, which induces alterations in cortical development and metabolic processes in astrocytes, among other cellular functions. These changes could modulate seizure susceptibility, contributing to the progression of epilepsy and its associated comorbidities. Epilepsy is characterized by dysregulation of calcium (Ca2+) channels and intracellular Ca2+ dynamics. These factors contribute to hyperexcitability, disrupted synaptogenesis, and altered synchronization of neuronal networks, all of which contribute to seizure activity. This study investigates the intricate interplay between altered Ca2+ dynamics, mTOR pathway dysregulation, and cellular metabolism in astrocytes. The transcriptional profile of TSC patients revealed significant alterations in pathways associated with cellular respiration, ER and mitochondria, and Ca2+ regulation. TSC astrocytes exhibited lack of responsiveness to various stimuli, compromised oxygen consumption rate and reserve respiratory capacity underscoring their reduced capacity to react to environmental changes or cellular stress. Furthermore, our study revealed significant reduction of store operated calcium entry (SOCE) along with strong decrease of basal mitochondrial Ca2+ concentration and Ca2+ influx in TSC astrocytes. In addition, we observed alteration in mitochondrial membrane potential, characterized by increased depolarization in TSC astrocytes. Lastly, we provide initial evidence of structural abnormalities in mitochondria within TSC patient-derived astrocytes, suggesting a potential link between disrupted Ca2+ signaling and mitochondrial dysfunction. Our findings underscore the complexity of the relationship between Ca2+ signaling, mitochondria dynamics, apoptosis, and mTOR hyperactivation. Further exploration is required to shed light on the pathophysiology of TSC and on TSC associated neuropsychiatric disorders offering further potential avenues for therapeutic development.

• Authors: Alessia Romagnolo, Giulia Dematteis, Mirte Scheper, Mark J Luinenburg, Angelika Mühlebner, Wim Van Hecke, Marcello Manfredi, Veronica De Giorgis, Simone Reano, Nicoletta Filigheddu, Valeria Bortolotto, Laura Tapella, Jasper J Anink, Liesbeth François, Stefanie Dedeurwaerdere, James D Mills, Armando A Genazzani, Dmitry Lim, Eleonora Aronica
• Journal: Acta neuropathologica (Acta Neuropathol) Vol. 147 Issue 1 Pg. 48 (Feb 28 2024) ISSN: 1432-0533 [Electronic] Germany
• PMID: 38418708 (Publication Type: Journal Article)
• Copyright: © 2024. The Author(s).

Chemical References

• Calcium
• TOR Serine-Threonine Kinases

Topics

• Humans
• Astrocytes (pathology)
• Calcium Signaling
• Tuberous Sclerosis (pathology)
• Calcium (metabolism)
• TOR Serine-Threonine Kinases (metabolism)
• Epilepsy (genetics)
• Homeostasis
• Seizures