Abstract |
Cisplatin resistance remains a persistent challenge in cervical cancer (CC) treatment. Molecular biomarkers have garnered attention for their association with cisplatin resistance in various diseases. Long non-coding RNAs (lncRNAs) exert significant influence on CC development. This study explores the role of LOC644656 in regulating cisplatin resistance in CC. Parental and cisplatin-resistant CC cells underwent cisplatin treatment. Functional assays assessed cell proliferation and apoptosis under different conditions. RNA pull-down with mass spectrometry, along with literature review, elucidated the interaction between LOC644656, ZNF143, and E6-AP. Mechanistic assays analyzed the relationship between different factors. RT-qPCR and western blot quantified RNA and protein levels, respectively. In vivo models validated E6-AP's function. Results revealed LOC644656 overexpression in cisplatin-resistant CC cells, exacerbating cell growth. LOC644656 recruited ZNF143 to activate E6-AP transcription, promoting cisplatin resistance in CC. In conclusion, LOC644656 positively modulates E6-AP expression via ZNF143-mediated transcriptional activation, contributing to cisplatin resistance in CC.
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Authors | Min Li, Jie Chen, Hong Zhang, Yi Zhang, Jiahui Wang, Zongji Shen, Youguo Chen, Wenjie Hou, Chi Chi |
Journal | Cellular signalling
(Cell Signal)
Vol. 117
Pg. 111115
(05 2024)
ISSN: 1873-3913 [Electronic] England |
PMID | 38395183
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2024 Elsevier Inc. All rights reserved. |
Chemical References |
- Cisplatin
- MicroRNAs
- RNA
- Trans-Activators
- ZNF143 protein, human
- UBE3A protein, human
- Ubiquitin-Protein Ligases
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Topics |
- Female
- Humans
- Cell Line, Tumor
- Cell Proliferation
- Cisplatin
(therapeutic use)
- Gene Expression Regulation, Neoplastic
- MicroRNAs
(metabolism)
- RNA
- Trans-Activators
(metabolism)
- Transcriptional Activation
- Uterine Cervical Neoplasms
(drug therapy, genetics)
- Drug Resistance, Neoplasm
- Ubiquitin-Protein Ligases
(metabolism)
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