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LOC644656 promotes cisplatin resistance in cervical cancer by recruiting ZNF143 and activating the transcription of E6-AP.

Abstract
Cisplatin resistance remains a persistent challenge in cervical cancer (CC) treatment. Molecular biomarkers have garnered attention for their association with cisplatin resistance in various diseases. Long non-coding RNAs (lncRNAs) exert significant influence on CC development. This study explores the role of LOC644656 in regulating cisplatin resistance in CC. Parental and cisplatin-resistant CC cells underwent cisplatin treatment. Functional assays assessed cell proliferation and apoptosis under different conditions. RNA pull-down with mass spectrometry, along with literature review, elucidated the interaction between LOC644656, ZNF143, and E6-AP. Mechanistic assays analyzed the relationship between different factors. RT-qPCR and western blot quantified RNA and protein levels, respectively. In vivo models validated E6-AP's function. Results revealed LOC644656 overexpression in cisplatin-resistant CC cells, exacerbating cell growth. LOC644656 recruited ZNF143 to activate E6-AP transcription, promoting cisplatin resistance in CC. In conclusion, LOC644656 positively modulates E6-AP expression via ZNF143-mediated transcriptional activation, contributing to cisplatin resistance in CC.
AuthorsMin Li, Jie Chen, Hong Zhang, Yi Zhang, Jiahui Wang, Zongji Shen, Youguo Chen, Wenjie Hou, Chi Chi
JournalCellular signalling (Cell Signal) Vol. 117 Pg. 111115 (05 2024) ISSN: 1873-3913 [Electronic] England
PMID38395183 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2024 Elsevier Inc. All rights reserved.
Chemical References
  • Cisplatin
  • MicroRNAs
  • RNA
  • Trans-Activators
  • ZNF143 protein, human
  • UBE3A protein, human
  • Ubiquitin-Protein Ligases
Topics
  • Female
  • Humans
  • Cell Line, Tumor
  • Cell Proliferation
  • Cisplatin (therapeutic use)
  • Gene Expression Regulation, Neoplastic
  • MicroRNAs (metabolism)
  • RNA
  • Trans-Activators (metabolism)
  • Transcriptional Activation
  • Uterine Cervical Neoplasms (drug therapy, genetics)
  • Drug Resistance, Neoplasm
  • Ubiquitin-Protein Ligases (metabolism)

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