Mechanism of LncRNA FTX regulates nephroblastoma progression through MiR-215-5p/PI3K/AKT axis.

Abstract

BACKGROUND:

Nephroblastoma, also more commonly known as Wilms tumor (WT), is a common childhood malignancy that connects tumorigenesis and organ development in the kidney.

OBJECTIVE:

The current study focused on the effect of lncRNA FTX in nephroblastoma.

STUDY DESIGN:

Expression of lncRNA FTX in nephroblastoma tissues and cells was determined. The expression location of lncRNA FTX was detected by FISH. The binding of lncRNA FTX and miR-215-5p with Ago2 was verified by RIP. Following gain- and loss-of-function approaches, the crucial role of lncRNA FTX and miR-215-5p in nephroblastoma cell functions was measured with the involvement of the PI3K/AKT pathway.

RESULTS:

LncRNA FTX was elevated and miR-215-5p was declined in nephroblastoma. Silencing of lncRNA FTX or mimic of miR-215-5p inhibited the malignant properties of nephroblastoma cells. LncRNA FTX was localized in the cytoplasm and might bind miR-215-5p. LncRNA FTX promoted the malignant features of nephroblastoma cells by inhibiting miR-215-5p through activating of the PI3K/AKT pathway.

CONCLUSIONS:

LncRNA FTX is capable of accelerating nephroblastoma development in vitro by reducing miR-215-5p through activating of the PI3K/AKT pathway, indicating LncRNA FTX may possibly a future target for the diagnosis and treatment of nephroblastoma. SUMMARY FIGURE.

• Authors: Li Wang, Qin Huang, Hui Li, Haisha Li, Xiangyun Wang, Xin Tan
• Journal: Journal of pediatric urology (J Pediatr Urol) (Jan 28 2024) ISSN: 1873-4898 [Electronic] England
• PMID: 38365477 (Publication Type: Journal Article)
• Copyright: Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.