Production of bilirubin via whole-cell transformation utilizing recombinant Corynebacterium glutamicum expressing a β-glucuronidase from Staphylococcus sp. RLH1.

Abstract

Bilirubin, a key active ingredient of bezoars with extensive clinical applications in China, is produced through a chemical process. However, this method suffers from inefficiency and adverse environmental impacts. To address this challenge, we present a novel and efficient approach for bilirubin production via whole-cell transformation. In this study, we employed Corynebacterium glutamicum ATCC13032 to express a β-glucuronidase (StGUS), an enzyme from Staphylococcus sp. RLH1 that effectively hydrolyzes conjugated bilirubin to bilirubin. Following the optimization of the biotransformation conditions, a remarkable conversion rate of 79.7% in the generation of bilirubin was obtained at temperate 40 °C, pH 7.0, 1 mM Mg2+ and 6 mM antioxidant NaHSO3 after 12 h. These findings hold significant potential for establishing an industrially viable platform for large-scale bilirubin production.

• Authors: Wei Zhou, Yanan Cui, Mengyun Chen, Qijun Gao, Kai Bao, Yongzhong Wang, Min Zhang
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 46 Issue 2 Pg. 223-233 (Apr 2024) ISSN: 1573-6776 [Electronic] Netherlands
• PMID: 38310624 (Publication Type: Journal Article)
• Copyright: © 2024. The Author(s), under exclusive licence to Springer Nature B.V.

Chemical References

• Bilirubin
• Glucuronidase

Topics

• Bilirubin
• Glucuronidase (genetics, metabolism)
• Corynebacterium glutamicum (metabolism)
• Staphylococcus
• China