Abstract | Background: Methods: A peptide from the Japanese encephalitis virus (JEV), which shares a similar amino acid sequence with GluN1, was identified by sequence comparison. We then explored whether active subcutaneous immunization with the JEV peptide could induce the production of anti-NMDAR antibodies and related pathophysiological and behavioral changes in mice. In addition, a published active immune model of anti-NMDAR encephalitis using a GluN1 peptide was used as the positive control. Results: A 6-amino-acid sequence with 83 % similarity between the envelope protein of the JEV (HGTVVI) and GluN1 (NGTHVI) was identified, and the sequence included the N368/G369 region. Active immunization with the JEV peptide induced a substantial and specific immune response in mice. However, anti-NMDAR antibodies were not detected in the serum of mice immunized with the JEV peptide by ELISA, CBA, and TBA. Moreover, mice immunized with the JEV peptide presented no abnormities related to anti-NMDAR antibodies according to western blotting, patch clamp, and a series of behavioral tests. In addition, active immunization with a recently reported GluN1 peptide failed to induce anti-NMDAR antibody production in mice. Conclusions:
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Authors | Hanyu Luo, Jiaxin Yang, Xiaoyue Yang, Ziyao Han, Zhixu Fang, Dishu Huang, Jianxiong Gui, Ran Ding, Hengsheng Chen, Li Cheng, Jiannan Ma, Li Jiang |
Journal | Heliyon
(Heliyon)
Vol. 10
Issue 2
Pg. e24700
(Jan 30 2024)
ISSN: 2405-8440 [Print] England |
PMID | 38298637
(Publication Type: Journal Article)
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Copyright | © 2024 The Authors. |