Abstract | BACKGROUND: Active components from natural fungal products have shown promising potential as anti- tumor therapeutic agents. In the search for anti- tumor agents, research to overcome the drawbacks of high molecular weight and low bioavailability of pure polysaccharides, polysaccharide-conjugated selenium nanoparticles (SeNPs) has attracted much attention. RESULTS: A novel polysaccharide- selenium nanoparticle complex was produced, in which SeNPs were decorated with polysaccharide obtained from fermented mycelia broth of Lactarius deliciosus (FLDP). Transmission electron microscope, dynamic light scattering, and X-ray photoelectron spectroscopy were utilized to characterize the FLDP-SeNPs; and human hepatocarcinoma cell line (HepG2) was used to assess growth inhibition efficacy. The FLDP-SeNPs that were prepared had a spherical shape with the smallest mean diameter of 32 nm. The FLDP-SeNPs showed satisfactory dispersibility and stability after combination, demonstrating that a reliable consolidated structure had formed. The results revealed that FLDP-SeNPs had notable growth inhibition effects on HepG2 cells. They reduced the membrane potential of mitochondria significantly, increased the generation of reactive oxygen species, enhanced levels of both Caspase-3 and Caspase-9, and led to the nucleus in a wrinkled form. CONCLUSION: The FLDP-SeNPs could exert a synergetic toxicity reduction and inhibition enhancement effect on HepG2 cells by inducing early apoptosis, through mitochondria-mediated cytochrome C- Caspases and reactive oxygen species-induced DNA damage pathways. These results indicate that FLDP- SeNP treatment of HepG2 cells induced early apoptosis with synergetic efficacy, showing that FLDP-SeNPs can be useful as natural anti- tumor agents. © 2024 Society of Chemical Industry.
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Authors | Qingxi Wu, Xiaohui Wang, Siwei Hao, Yingchao Wu, Wenna Zhang, Lei Chen, Chao Yan, Yongming Lu, Yan Chen, Zhifeng Ding |
Journal | Journal of the science of food and agriculture
(J Sci Food Agric)
(Jan 29 2024)
ISSN: 1097-0010 [Electronic] England |
PMID | 38284440
(Publication Type: Journal Article)
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Copyright | © 2024 Society of Chemical Industry. |