The discovery of the interactome of
cannabidiol (CBD), a non-psychoactive
cannabinoid from Cannabis sativa L., has been here performed on
chronic myelogenous leukemia cancer cells, using an optimized chemo-proteomic stage, which links Drug Affinity Responsive Target Stability with Limited Proteolysis Multiple Reaction Monitoring approaches. The obtained results showed the ability of CBD to target simultaneously some potential
protein partners, corroborating its well-known poly-pharmacology activity. In human
chronic myelogenous leukemia K562
cancer cells, the most fascinating
protein partner was identified as the 116 kDa
U5 small nuclear ribonucleoprotein element called EFTUD2, which fits with the spliceosome complex. The binding mode of this oncogenic
protein with CBD was clarified using mass spectrometry-based and in silico analysis.