Abstract | AIM: MATERIALS AND METHODS: PTX3 contents of early and advanced atherosclerotic lesions of the aorta obtained at autopsy were determined by ELISA and Western blot. Also, coronary plaques of patients with acute coronary syndrome (ACS) or stable angina pectoris (SAP) obtained by DCA were analyzed by immunohistochemistry for PTX3. The effects of PTX3 on smooth muscle cells (SMCs) and thrombogenesis were investigated with cultured human coronary artery SMCs and a flow chamber system, respectively. RESULTS: Advanced atherosclerotic lesions contained a significantly larger amount of PTX3 than early lesions (ELISA: 9.96 ± 2.77 ng/100 mg tissue, n = 8 vs 0.24 ± 0.18 ng/100 mg tissue, n = 6, P = 0.0097). Also, ACS plaques contained a significantly larger amount of PTX3 than SAP plaques (PTX3 immunohistochemistry-positive area percentage: 2.88 ± 0.53 %, n = 22 vs 0.67 ± 0.27 %, n = 23, P = 0.0009). Curiously, the patients who would remain free of post-DCA restenosis (n = 19) had plaques with a significantly higher PTX3 immunohistochemistry-positive area percentage than those who would develop restenosis (n = 12) (2.32 ± 0.49 % vs 0.49 ± 0.17 %, P = 0.002). In the mechanistic part of the study, PTX3 inhibited SMC proliferation and migration. PTX3 also inhibited platelet thrombus formation in the condition simulating arterial blood flow. CONCLUSIONS: PTX3 is increased in advanced (vs early) atherosclerotic lesions and unstable (vs stable) coronary plaques. The inhibitory effects of PTX3 on SMCs and thrombogenesis suggest that intraplaque PTX3 might have atheroprotective effects.
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Authors | Tomoyuki Otani, Sayaka Moriguchi-Goto, Kensaku Nishihira, Nobuyuki Oguri, Yoshisato Shibata, Yunosuke Matsuura, Tatsuhiko Kodama, Yujiro Asada, Kinta Hatakeyama, Atsushi Yamashita |
Journal | Thrombosis research
(Thromb Res)
Vol. 234
Pg. 134-141
(02 2024)
ISSN: 1879-2472 [Electronic] United States |
PMID | 38218110
(Publication Type: Journal Article)
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Copyright | Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved. |
Chemical References |
- PTX3 protein
- C-Reactive Protein
- Serum Amyloid P-Component
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Topics |
- Humans
- Atherosclerosis
- C-Reactive Protein
(analysis)
- Plaque, Atherosclerotic
- Thrombosis
(etiology, prevention & control)
- Disease Progression
- Acute Coronary Syndrome
- Coronary Artery Disease
- Serum Amyloid P-Component
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