Abstract |
ATP and its receptor P2RX7 exert a pivotal effect on antitumor immunity during chemotherapy-induced immunogenic cell death (ICD). Here, we demonstrated that TNFα-mediated PANX1 cleavage was essential for ATP release in response to chemotherapy in colorectal cancer (CRC). TNFα promoted PANX1 cleavage via a caspase 8/3-dependent pathway to enhance cancer cell immunogenicity, leading to dendritic cell maturation and T-cell activation. Blockade of the ATP receptor P2RX7 by the systemic administration of small molecules significantly attenuated the therapeutic efficacy of chemotherapy and decreased the infiltration of immune cells. In contrast, administration of an ATP mimic markedly increased the therapeutic efficacy of chemotherapy and enhanced the infiltration of immune cells in vivo. High PANX1 expression was positively correlated with the recruitment of DCs and T cells within the tumor microenvironment and was associated with favorable survival outcomes in CRC patients who received adjuvant chemotherapy. Furthermore, a loss-of-function P2RX7 mutation was associated with reduced infiltration of CD8+ immune cells and poor survival outcomes in patients. Taken together, these results reveal that TNFα-mediated PANX1 cleavage promotes ATP-P2RX7 signaling and is a key determinant of chemotherapy-induced antitumor immunity.
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Authors | Kevin Chih-Yang Huang, Shu-Fen Chiang, Pei-Chun Lin, Wei-Ze Hong, Pei-Chen Yang, Hui-Ping Chang, Shin-Lei Peng, Tsung-Wei Chen, Tao-Wei Ke, Ji-An Liang, William Tzu-Liang Chen, K S Clifford Chao |
Journal | Cell death & disease
(Cell Death Dis)
Vol. 15
Issue 1
Pg. 24
(01 09 2024)
ISSN: 2041-4889 [Electronic] England |
PMID | 38195677
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2024. The Author(s). |
Chemical References |
- Tumor Necrosis Factor-alpha
- Antineoplastic Agents
- Adenosine Triphosphate
- PANX1 protein, human
- Nerve Tissue Proteins
- Connexins
- P2RX7 protein, human
- Receptors, Purinergic P2X7
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Topics |
- Humans
- Tumor Necrosis Factor-alpha
- Lymphocyte Activation
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Colorectal Neoplasms
(drug therapy, genetics)
- Adenosine Triphosphate
- Tumor Microenvironment
- Nerve Tissue Proteins
- Connexins
(genetics)
- Receptors, Purinergic P2X7
(genetics)
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