Ferroptosis is a new way of cell death which is reported to participate in the pathology of
myocardial ischemia-reperfusion (MI/R) injury, but it's mechanism remains unclear. The present investigation is to study the emerging role of
long non-coding RNA (
lncRNA) regulator of reprogramming (ROR) in cardiomyocyte ferroptosis after
hypoxia/reoxygenation (H/R) administration. RT-qPCR and/or Western blot methods were performed to examine the gene/or
protein levels, and
CCK-8, ELISA, and
DCFH-DA staining determined the cellular viability and ferroptosis. Dual-
luciferase and
RNA immunoprecipitation were applied to verify molecular interaction.
LncRNA ROR and miR-769-5p were overexpressed and reduced in blood samples from MI patients and H/R-treated AC16 cells, respectively. Mechanistically, lncROR sponged to miR-769-5p, thus upregulating CBX7 expression. Functional experiments presented that
lncRNA ROR silence mitigated H/R-stimulated inflammatory damage, oxidative stress, and ferroptosis in AC16 cells, whereas these roles could be reversed by co-downregulation of miR-769-5p or co-overexpression of CBX7. These data uncovered that
lncRNA ROR prevented against H/R-induced cardiomyocyte ferroptosis by modulating miR-769-5p/CBX7 signaling, emphasizing the therapeutic value of
lncRNA ROR in MI/R injury.