Abstract | OBJECTIVE: To assess whether bleaching gel volume influences chromatic changes, hydrogen peroxide (HP) diffusion, inflammation, and oxidative stress in the pulp tissue. METHODOLOGY: A total of 60 bovine teeth were divided into four groups, according to bleaching gel volume (n=15): without gel (WG); V30 (30 µL of 35% HP); V60 (60 µL); and V120 (120 μL). HP diffusion analysis was performed in the first session (T1). Chromatic changes (ΔE, ΔE00, and WID) were assessed after the first (T1), second (T2), third (T3) sessions, and 15 d (T4) after the end of treatment. Moreover, 20 rats were randomly divided into four groups (n=10) and their upper first molars were treated with different gel volumes: control (no treatment); V2 (2 μL of 17.5% HP); V4 (4 μL); and V8 (8 μL). After 24 h, rats were euthanized and the specimens processed for histological and immunohistochemical ( nitric oxide synthase) evaluation. Data were analyzed using the Wilcoxon and Mann-Whitney tests (p<0.05). RESULTS: In vitro (bovine teeth), chromatic changes were not influenced by bleaching gel volume, showing similar values in all groups and sessions, except for the control group (p<0.05). The V120 group had the highest HP diffusion values (p<0.05). In vivo (pulp tissue), the V4 and V8 groups showed the highest inflammatory infiltrate in the pulp and significant oxidative stress (p<0.05). CONCLUSION: The adverse effects on the dental pulp related to HP diffusion, pulp inflammation, and oxidative stress depend on bleaching gel volume, while the bleaching effect is not proportional to the volume used.
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Authors | Sibele de Alcântara, Francine Benetti, Lívia Maria Alves Valentim da Silva, Nathália Evelyn da Silva Machado, Isabela Joane Prado Silva, Lara Maria Bueno Esteves, Edilson Ervolino, Luciano Tavares Angelo Cintra, André Luiz Fraga Briso |
Journal | Journal of applied oral science : revista FOB
(J Appl Oral Sci)
Vol. 31
Pg. e20230265
( 2023)
ISSN: 1678-7765 [Electronic] Brazil |
PMID | 38126578
(Publication Type: Journal Article)
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Chemical References |
- Hydrogen Peroxide
- Nitric Oxide
- Tooth Bleaching Agents
- Anti-Infective Agents
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Topics |
- Animals
- Cattle
- Rats
- Hydrogen Peroxide
(adverse effects)
- Tooth Bleaching
- Nitric Oxide
- Tooth Bleaching Agents
(adverse effects)
- Anti-Infective Agents
- Inflammation
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