CAZymes-associated method to explore glycans that mitigate DSS-induced colitis via targeting Bacteroides cellulosilyticus.

Abstract

Improving Bacteroides cellulosilyticus abundance is a feasible approach to treating inflammatory bowel disease (IBD). Although B. cellulosilyticus is responsive to dietary components, untargeted manipulation cannot focus on target microbe and lead to an increase in harmful bacteria in the microbiota. Breakthroughs in methods for regulating specific microbes, but the protocols are expensive, time-consuming, and difficult to follow. Glycans based on microbial-carbohydrate-active enzymes (CAZymes) would provide a potential solution. We propose a method based on CAZymes to explore polysaccharides that target specific gut microbes and alleviate diseases. The designed polysaccharides (Arabinogalactan, AG) enrich the abundance of B. cellulosilyticus in single-strain co-cultures, fermentation in vitro, and mouse models in vivo. Supplementation with AG relieved mice from colitis and clinical symptoms. We reveal that AG directly alters B. cellulosilyticus level and cooperative microbes, resulting in remission of colitis. Our glycan design pipeline is a promising way to improve disease through the targeted enhancement of specific microbes.

• Authors: Xiaoxiang Gao, FuSheng Xu, Tangjun Li, Pan Huang, Leilei Yu, Fengwei Tian, Jianxin Zhao, Wei Chen, Qixiao Zhai
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 258 Issue Pt 1 Pg. 128694 (Feb 2024) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 38096941 (Publication Type: Journal Article)
• Copyright: Copyright © 2023 Elsevier B.V. All rights reserved.

Chemical References

• Polysaccharides
• Dextran Sulfate

Topics

• Animals
• Mice
• Gastrointestinal Microbiome (physiology)
• Colitis (drug therapy)
• Bacteroides
• Polysaccharides (therapeutic use)
• Dextran Sulfate (adverse effects)
• Disease Models, Animal
• Mice, Inbred C57BL
• Colon (microbiology)