Balance between the CMC/ACP Nanocomplex and Blood Assimilation Orchestrates Immunomodulation of the Biomineralized Collagen Matrix.

Abstract

Calcium phosphate-based biomineralized biomaterials have broad application prospects. However, the immune response and foreign body reactions elicited by biomineralized materials have drawn substantial attention recently, contrary to the immune microenvironment optimization concept. Therefore, it is important to clarify the immunomodulation properties of biomineralized materials. Herein, we prepared the biomineralized collagen matrix (BCM) and screened the key immunomodulation factor carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) nanocomplex. The immunomodulation effect of the BCM was investigated in vitro and in vivo. The BCM triggered evident inflammatory responses and cascade foreign body reactions by releasing the CMC/ACP nanocomplex, which activated the potential TLR4-MAPK/NF-κB pathway, compromising the collagen matrix biocompatibility. By contrast, blocking the CMC/ACP nanocomplex release via the blood assimilation process of the BCM mitigated the inflammation and foreign body reactions, enhancing biocompatibility. Hence, the immunomodulation of the BCM was orchestrated by the balance between the CMC/ACP nanocomplex and the blood assimilation process. Controlling the release of the CMC/ACP nanocomplex to accord the biological effects of ACP with the temporal regenerative demands is key to developing advanced biomineralized materials.

• Authors: Mengxi Su, Chuangji Li, Shudan Deng, Leyao Xu, Zhengjie Shan, Yihan Xing, Xiyan Li, Ye Li, Xingchen Liu, Xinyi Zhong, Kaidi Chen, Shoucheng Chen, Quan Liu, Xiayi Wu, Zetao Chen, Shiyu Wu, Zhuofan Chen
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 15 Issue 50 Pg. 58166-58180 (Dec 20 2023) ISSN: 1944-8252 [Electronic] United States
• PMID: 38079631 (Publication Type: Journal Article)

Chemical References

• Collagen
• Biocompatible Materials
• NF-kappa B
• Calcium Phosphates

Topics

• Humans
• Collagen
• Biocompatible Materials (pharmacology)
• NF-kappa B
• Immunity
• Foreign Bodies
• Calcium Phosphates