Liquid Biopsy in Organ Damage: small extracellular vesicle chip-based assessment of polytrauma.

Abstract

Background:

Despite major advances in medicine, blood-borne biomarkers are urgently needed to support decision-making, including polytrauma. Here, we assessed serum-derived extracellular vesicles (EVs) as potential markers of decision-making in polytrauma.

Objective:

Our Liquid Biopsy in Organ Damage (LiBOD) study aimed to differentiate polytrauma with organ injury from polytrauma without organ injury. We analysed of blood-borne small EVs at the individual level using a combination of immunocapture and high-resolution imaging.

Methods:

To this end, we isolated, purified, and characterized small EVs according to the latest Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines from human blood collected within 24 h post-trauma and validated our results using a porcine polytrauma model.

Results:

We found that small EVs derived from monocytes CD14+ and CD14+CD61+ were significantly elevated in polytrauma with organ damage. To be precise, our findings revealed that CD9+CD14+ and CD14+CD61+ small EVs exhibited superior performance compared to CD9+CD61+ small EVs in accurately indicating polytrauma with organ damage, reaching a sensitivity and a specificity of 0.81% and 0.97%, respectively. The results in humans were confirmed in an independent porcine model of polytrauma.

Conclusion:

These findings suggest that these specific types of small EVs may serve as valuable, non-invasive, and objective biomarkers for assessing and monitoring the severity of polytrauma and associated organ damage.

• Authors: Bingduo Wang, Aliona Wöhler, Johannes Greven, Rebekka J S Salzmann, Cindy M Keller, Tobias Tertel, Qun Zhao, Ümit Mert, Klemens Horst, Ludmila Lupu, Markus Huber-Lang, Martijn van Griensven, Tom Erik Mollnes, Sebastian Schaaf, Robert Schwab, Christian P Strassburg, Ingo G H Schmidt-Wolf, Bernd Giebel, Frank Hildebrand, Veronika Lukacs-Kornek, Arnulf G Willms, Miroslaw T Kornek
• Journal: Frontiers in immunology (Front Immunol) Vol. 14 Pg. 1279496 ( 2023) ISSN: 1664-3224 [Electronic] Switzerland
• PMID: 38035093 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 Wang, Wöhler, Greven, Salzmann, Keller, Tertel, Zhao, Mert, Horst, Lupu, Huber-Lang, van Griensven, Mollnes, Schaaf, Schwab, Strassburg, Schmidt-Wolf, Giebel, Hildebrand, Lukacs-Kornek, Willms and Kornek.

Chemical References

• Biomarkers

Topics

• Humans
• Animals
• Swine
• Extracellular Vesicles (pathology)
• Biomarkers
• Liquid Biopsy
• Monocytes
• Multiple Trauma (pathology)