Click-Chemistry-Mediated Cell Membrane Glycopolymer Engineering to Potentiate Dendritic Cell Vaccines.

Abstract

Dendritic cell vaccine (DCV) holds great potential in tumor immunotherapy owing to its potent ability in eliciting tumor-specific immune responses. Aiming at engineering enhanced DCV, we report the first effort to construct a glycopolymer-engineered DC vaccine (G-DCV) via metabolicglycoengineering and copper-free click-chemistry. Model G-DCV was prepared by firstly delivering tumor antigens, ovalbumin (OVA) into dendritic cells (DC) with fluoroalkane-grafted polyethyleneimines, followed by conjugating glycopolymers with a terminal group of dibenzocyclooctyne (DBCO) onto dendritic cells. Compared to unmodified DCV, our G-DCV could induce stronger T cell activation due to the enhanced adhesion between DCs and T cells. Notably, such G-DCV could more effectively inhibit the growth of the mouse B16-OVA (expressing OVA antigen) tumor model after adoptive transfer. Moreover, by combination with an immune checkpoint inhibitor, G-DCV showed further increased anti-tumor effects in treating different tumor models. Thus, our work provides a novel strategy to enhance the therapeutic effectiveness of DC vaccines.

• Authors: He Yang, Zijian Xiong, Xingyu Heng, Xiaomeng Niu, Yichen Wang, Lihua Yao, Lele Sun, Zhuang Liu, Hong Chen
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 63 Issue 2 Pg. e202315782 (Jan 08 2024) ISSN: 1521-3773 [Electronic] Germany
• PMID: 38018480 (Publication Type: Journal Article)
• Copyright: © 2023 Wiley-VCH GmbH.

Chemical References

• Antigens, Neoplasm
• Ovalbumin
• Vaccines

Topics

• Mice
• Animals
• T-Lymphocytes
• Antigens, Neoplasm
• Neoplasms (metabolism)
• Ovalbumin
• Cell Membrane
• Dendritic Cells (metabolism)
• Vaccines