Sleep apnea syndrome (SAS) exposes cells throughout the body to intermittent
hypoxia (IH). Intermittent
hypoxia is a risk factor not only for
hypertension and
insulin resistance but also for vascular dysfunction. We have reported correlations between IH,
insulin resistance and
hypertension. However, the details of why IH leads to vascular dysfunction remain unclear. In this study, we investigated
inflammation-related transcripts in vascular endothelial cells (human HUEhT-1 and mouse UV2) exposed to IH by real-time RT-PCR and found that
intercellular adhesion molecule-1 (ICAM-1) and endothelial cell-specific molecule-1 (ESM1) mRNAs were significantly increased. ELISA confirmed that, in the UV2 cell medium,
ICAM-1 and ESM1 were significantly increased by IH. However, the promoter activities of
ICAM-1 and ESM1 were not upregulated. On the other hand, IH treatment significantly decreased
microRNA (miR)-181a1 in IH-treated cells. The introduction of miR-181a1 mimic but not miR-181a1 mimic NC abolished the IH-induced upregulation of Ican-1 and ESM1. These results indicated that
ICAM-1 and ESM1 were upregulated by IH via the IH-induced downregulation of miR-181a1 in vascular endothelial cells and suggested that SAS patients developed
atherosclerosis via the IH-induced upregulation of
ICAM-1 and ESM1.