Abstract | Background: Methods: Western blot and quantitative reverse transcription polymerase chain reaction (RT-qPCR) were employed to ascertain the protein and gene levels of IL-1β, IL-6, and TNF-α in BV2 cells. The concentration of IL-6 in the supernatant of the BV2 cell culture was measured by enzyme-linked immunosorbent assay. For the assessment of mitochondrial homeostasis, western blot, RT-qPCR, and cellular immunohistochemistry methods were utilized to investigate the protein and gene levels of DRP1 and MFN-2 in HT22 cells. In the context of signal pathway analyses, western blot, RT-qPCR, and immunofluorescence techniques were employed to detect the protein and gene expressions of PGC-1α and SIRT3 in HT22 cells, respectively. Following the transfection with siPGC-1αRNA, downstream proteins of PGC-1α/ SIRT3 pathway in HT22 cells were investigated by Western blot and RT-qPCR. Results: The experimental findings demonstrated that exposure to PM2.5 exacerbated neuroinflammation, resulting in elevated levels of IL-1β, IL-6, and TNF-α. Furthermore, it perturbed mitochondrial dynamics, as evidenced by increased DRP1 expression and decreased MFN-2 expression. Additionally, dysfunction was observed in the PGC-1α/ SIRT3 signal pathway. However, intervention with SVHRSP ameliorated the cellular damage induced by PM2.5 exposure. Conclusions: SVHRSP alleviated neuroinflammation and mitochondrial dynamics imbalance induced by PM2.5 exposure by downregulating the PGC-1α/ SIRT3 signaling pathway.
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Authors | Lanyi Huang, Jingbin Xu, Kaiqian Duan, Tuya Bao, Yu Cheng, Haimin Zhang, Yong Zhang, Yingwei Lin, Fasheng Li |
Journal | Toxicology research
(Toxicol Res (Camb))
Vol. 12
Issue 5
Pg. 756-764
(Oct 2023)
ISSN: 2045-452X [Print] England |
PMID | 37915494
(Publication Type: Journal Article)
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Copyright | © The Author(s) 2023. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]. |