PVA enema ameliorates DSS-induced acute colitis in mice.

Abstract

BACKGROUND:

Ulcerative colitis (UC) represents a clinically challenging condition characterized by persistent damage to the colonic epithelial mucosa as the principal pathological feature. Polyvinyl alcohol (PVA) solution, primarily composed of glue, is a biodegradable polymer material that has found utility in the medical field. This research endeavors to investigate the therapeutic potential of PVA water solution in ameliorating UC in mice.

METHODS:

UC was induced in 48 C57BL/6 mice by administering 2.5% DSS in their diet for 6 days. Mice were treated with different concentrations of PVA (0.1 mg/ml PVA, 0.3 mg/ml PVA, 1 mg/ml PVA, 3 mg/ml PVA, 10 mg/ml PVA) enemas (n = 6). Disease Activity Index (DAI) and histologic score were evaluated for inflammation degree. Furthermore, mouse colon organoids were cultured, which were used to assess the effects of PVA on expansion in vitro.

RESULTS:

PVA aqueous solutions (1 mg/ml and 3 mg/ml) were able to alleviate the DAI in mice. By DAY 6, there was a significant 3/5-fold decrease in DAI within the 1 mg/ml PVA group (p = 0.02). Histopathology scores demonstrated improvements, while the levels of inflammatory factors in the intestinal mucosal tissue were reduced. Additionally, it was confirmed that PVA could promote the expansion of colonic organoids in vitro.

CONCLUSIONS:

In summary, our investigation has yielded findings indicating that PVA holds the potential to ameliorate symptoms associated with colitis in murine subjects afflicted by DSS-induced colitis, primarily through its facilitation of intestinal stem cell expansion. This study might provide a new candidate for the clinical treatment of ulcerative colitis.

• Authors: Zhiyong Zhang, Lingnan Kong, Ming Lv, Yukuan Yao, Li Gao, Ruchen Zhou, Wenlong Ma, Jing Li
• Journal: BMC gastroenterology (BMC Gastroenterol) Vol. 23 Issue 1 Pg. 368 (Oct 30 2023) ISSN: 1471-230X [Electronic] England
• PMID: 37904100 (Publication Type: Journal Article)
• Copyright: © 2023. The Author(s).

Chemical References

• Polyvinyl Alcohol
• Dextran Sulfate

Topics

• Humans
• Mice
• Animals
• Colitis, Ulcerative (drug therapy)
• Polyvinyl Alcohol (adverse effects)
• Mice, Inbred C57BL
• Colitis (therapy, drug therapy)
• Colon (pathology)
• Enema
• Dextran Sulfate (adverse effects)
• Disease Models, Animal