A quinolin-8-ol sub-millimolar inhibitor of UGGT, the ER glycoprotein folding quality control checkpoint.

Abstract

Misfolded glycoprotein recognition and endoplasmic reticulum (ER) retention are mediated by the ER glycoprotein folding quality control (ERQC) checkpoint enzyme, UDP-glucose glycoprotein glucosyltransferase (UGGT). UGGT modulation is a promising strategy for broad-spectrum antivirals, rescue-of-secretion therapy in rare disease caused by responsive mutations in glycoprotein genes, and many cancers, but to date no selective UGGT inhibitors are known. The small molecule 5-[(morpholin-4-yl)methyl]quinolin-8-ol (5M-8OH-Q) binds a CtUGGTGT24 "WY" conserved surface motif conserved across UGGTs but not present in other GT24 family glycosyltransferases. 5M-8OH-Q has a 47 μM binding affinity for CtUGGTGT24in vitro as measured by ligand-enhanced fluorescence. In cellula, 5M-8OH-Q inhibits both human UGGT isoforms at concentrations higher than 750 μM. 5M-8OH-Q binding to CtUGGTGT24 appears to be mutually exclusive to M5-9 glycan binding in an in vitro competition experiment. A medicinal program based on 5M-8OH-Q will yield the next generation of UGGT inhibitors.

• Authors: Kevin P Guay, Roberta Ibba, J L Kiappes, Snežana Vasiljević, Francesco Bonì, Maria De Benedictis, Ilaria Zeni, James D Le Cornu, Mario Hensen, Anu V Chandran, Anastassia L Kantsadi, Alessandro T Caputo, Juan I Blanco Capurro, Yusupha Bayo, Johan C Hill, Kieran Hudson, Andrea Lia, Juliane Brun, Stephen G Withers, Marcelo Martí, Emiliano Biasini, Angelo Santino, Matteo De Rosa, Mario Milani, Carlos P Modenutti, Daniel N Hebert, Nicole Zitzmann, Pietro Roversi
• Journal: iScience (iScience) Vol. 26 Issue 10 Pg. 107919 (Oct 20 2023) ISSN: 2589-0042 [Electronic] United States
• PMID: 37822503 (Publication Type: Journal Article)
• Copyright: © 2023 The Author(s).