The onset of adenosine monophosphate-activated protein kinase activity on orthodontic tooth movement in rats with type 2 diabetes.

Abstract

Adenosine monophosphate-activated protein kinase (AMPK) plays pivotal roles in metabolic diseases including type 2 diabetes. However, the specific role of AMPK for orthodontic tooth movement in type 2 diabetes is unclear. In this study, a diabetic rat model was established through dietary manipulation and streptozocin injection. Examinations were conducted to select qualified type 2 diabetic rats. Then, an orthodontic device was applied to these rats for 0, 3, 7, or 14 days. The distance of orthodontic tooth movement and parameters of alveolar bone were analyzed by micro-computed tomography. Periodontal osteoclastic activity, inflammatory status, and AMPK activity were measured via histological analyses. Next, we repeated the establishment of diabetic rats to investigate whether change of AMPK activity was associated with orthodontic tooth movement under type 2 diabetes. The results showed that diabetic rats exhibited an exacerbated alveolar bone resorption, overactive inflammation, and decreased periodontal AMPK activity during orthodontic tooth movement. Injection of the AMPK agonist alleviated type 2 diabetes-induced periodontal inflammation and alveolar bone resorption, thus normalizing distance of orthodontic tooth movement. Our study indicates that type 2 diabetes decreases periodontal AMPK activity, leading to excessive inflammation elevating osteoclast formation and alveolar bone resorption, which could be reversed by AMPK activation.

• Authors: Danyuan Huang, Yuyu Li, Shuo Chen, Han Wang, Yukun Jiang, Yuanyuan Wei, Hengyi Lin, Shujuan Zou
• Journal: European journal of oral sciences (Eur J Oral Sci) 2023 Oct-Nov Vol. 131 Issue 5-6 Pg. e12955 ISSN: 1600-0722 [Electronic] England
• PMID: 37805702 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2023 Scandinavian Division of the International Association for Dental Research. Published by John Wiley & Sons Ltd.

Chemical References

• AMP-Activated Protein Kinases

Topics

• Rats
• Animals
• Diabetes Mellitus, Type 2 (complications)
• Diabetes Mellitus, Experimental
• Tooth Movement Techniques (methods)
• X-Ray Microtomography
• AMP-Activated Protein Kinases
• Alveolar Bone Loss (diagnostic imaging)
• Inflammation
• Periodontal Ligament