Transfusion-related acute lung injury (
TRALI) is one of the leading causes of transfusion-related fatalities and, to date, is without available
therapies. Here, we investigated the role of the
complement system in
TRALI. Murine anti-major histocompatibility complex class I
antibodies were used in
TRALI mouse models, in combination with analyses of plasma samples from patients with
TRALI. We found that in vitro complement activation was related to in vivo antibody-mediated
TRALI induction, which was correlated with increased macrophage trafficking from the lungs to the blood in a fragment crystallizable region (Fc)-dependent manner and that this was dependent on C5. Human
immunoglobulin G 1 variants of the murine
TRALI-inducing antibody 34-1-2S, either unable to activate
complement and/or bind to Fcγ receptors (FcγRs), revealed an essential role for the
complement system, but not for FcγRs, in the onset of 34-1-2S-mediated
TRALI in mice. In addition, we found high levels of complement activation in the plasma of patients with
TRALI (n = 53), which correlated with elevated neutrophil extracellular trap (NET) markers. In vitro we found that NETs could be formed in a murine, 2-hit model, mimicking
TRALI with
lipopolysaccharide and C5a stimulation. Collectively, this reveals a critical role of Fc-mediated complement activation in
TRALI, with a direct relation to macrophage trafficking from the lungs to the blood and an association with NET formation, suggesting that targeting the
complement system may be an attractive therapeutic approach for combating
TRALI.