Mechanism of Sophorae Flavescentis Radix (Kushen) in treating NSCLC: Insights from miRNA-mRNA network analysis.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Sophorae Flavescentis Radix (Kushen) is the primary herb component of Compound Kushen Injection (CKI), an approved clinical treatment for tumors. Despite CKI's widespread use, the underlying mechanisms of Kushen regarding microRNA-target and pathway remain unclear in non-small cell lung cancer (NSCLC).

AIM OF THE STUDY:

This study aimed to elucidate the crucial miRNAs-targets and pathways responsible for the Kushen's impact on NSCLC.

MATERIALS AND METHODS:

CCK8, colony formation, and apoptosis assays were performed to assess the effects of Kushen on NSCLC cells. Subsequently, we treated the A549 cell line with varying concentrations of Kushen to obtain mRNA and miRNA expression profiles. A DE (differentially expressed) miRNAs-DEGs network was then constructed to identify the critical miRNA-mRNA interaction influenced by Kushen. Furthermore, we performed clinical significance and prognosis analyses of hub genes to narrow down key genes and their corresponding miRNAs. Finally, the effects of Kushen on critical miRNA-mRNA interaction and related pathway were verified by in vitro and in vivo experiments.

RESULTS:

In this study, we initially demonstrated that Kushen significantly inhibited cell proliferation, suppressed colony formation, and induced apoptosis in the A549 cells, PC9 cells, and the A549 zebrafish xenograft model. Through expression profile analysis, a DE miRs-DEGs network was constructed with 16 DE miRs and 68 DEGs. Through the network analysis and expression validation, we found Kushen could significantly down-regulate miR-183-5p expression and up-regulate EGR1 expression. Additionally, Kushen affected the PTEN/Akt pathway, increasing PTEN expression and decreasing pAkt expression. Finally, matrine, the essential active compound of Kushen, also inhibited cell growth, induced apoptosis, and regulated miR-183-5p/EGR1 and PTEN/AKT pathway.

CONCLUSIONS:

Altogether, these findings supported the critical role of miR-183-5p/EGR1 and the PTEN/AKT pathway in the beneficial effects of Kushen on NSCLC, highlighting the therapeutic potential of Kushen in NSCLC treatment.

• Authors: Jing Li, Yixian Huang, Jiatong Xu, Huali Zuo, Yun Tang, Hsi-Yuan Huang, Jihang Chen, Yang-Chi-Dung Lin, Hsien-Da Huang
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 319 Issue Pt 3 Pg. 117232 (Jan 30 2024) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 37757992 (Publication Type: Journal Article)
• Copyright: Copyright © 2023. Published by Elsevier B.V.

Chemical References

• MicroRNAs
• kushen
• Proto-Oncogene Proteins c-akt
• Biological Products

Topics

• Humans
• Animals
• MicroRNAs (genetics)
• Carcinoma, Non-Small-Cell Lung (drug therapy, genetics)
• Proto-Oncogene Proteins c-akt
• Zebrafish
• Lung Neoplasms (drug therapy, genetics)
• Biological Products