Stem Cells from Human Exfoliated Deciduous Teeth Alleviate High-Altitude Cerebral Oedema by Shifting Microglial M1/M2 Polarisation.

Abstract

OBJECTIVE:

To explore the high-efficiency and low-risk prevention and treatment strategies for stem cells from human exfoliated deciduous teeth (SHED) for high-altitude cerebral oedema.

METHODS:

A low-pressure and low-oxygen tank mimicking high-altitude conditions was used to establish the high-altitude cerebral oedema animal model. The preventive effects of SHED for cerebral oedema were then evaluated by haematoxylin and eosin (H&E) and histological staining. In vitro, SHED was co-cultured with BV-2 to analyse the effects of SHED by western blot and immunofluorescence staining.

RESULTS:

SHED can prevent and treat cerebral oedema in a high altitude rat animal model. Mechanistically, SHED treatment can protect brain cells from apoptosis induced by high altitude condition. Moreover, SHED treatment can inhibit M1-type polarisation and promote M2-type polarisation of microglia cells via the suppression of hypoxia inducible factor (HIF)- 1α-mediated extracellular signal-regulated kinase (ERK) signalling activated in high altitude condition.

CONCLUSION:

SHED treatment can relieve high-altitude cerebral oedema via inhibiting HIF- 1α-mediated ERK signalling, which indicates that SHED is a promising alternative strategy to prevent and treat high-altitude cerebral oedema.

• Authors: Yi Ming Wang, Yi Kun Zhou, Chun Shan Han, Liu Jing Chen, Zi Meng Zhuang, Rui Li Yang, Wei Ran Li
• Journal: The Chinese journal of dental research (Chin J Dent Res) Vol. 26 Issue 3 Pg. 153-162 (Sep 21 2023) ISSN: 1867-5646 [Electronic] England
• PMID: 37732681 (Publication Type: Journal Article)

Chemical References

• Extracellular Signal-Regulated MAP Kinases

Topics

• Humans
• Animals
• Rats
• Brain Edema (etiology, therapy)
• Microglia
• Altitude
• Stem Cells
• Altitude Sickness
• Extracellular Signal-Regulated MAP Kinases
• Tooth, Deciduous