Single-Cell Lipidomics Using Analytical Flow LC-MS Characterizes the Response to Chemotherapy in Cultured Pancreatic Cancer Cells.

Abstract

In this work, we demonstrate the development and first application of nanocapillary sampling followed by analytical flow liquid chromatography-mass spectrometry for single-cell lipidomics. Around 260 lipids were tentatively identified in a single cell, demonstrating remarkable sensitivity. Human pancreatic ductal adenocarcinoma cells (PANC-1) treated with the chemotherapeutic drug gemcitabine can be distinguished from controls solely on the basis of their single-cell lipid profiles. Notably, the relative abundance of LPC(0:0/16:0) was significantly affected in gemcitabine-treated cells, in agreement with previous work in bulk. This work serves as a proof of concept that live cells can be sampled selectively and then characterized using automated and widely available analytical workflows, providing biologically relevant outputs.

• Authors: Kyle D G Saunders, Johanna von Gerichten, Holly-May Lewis, Priyanka Gupta, Matt Spick, Catia Costa, Eirini Velliou, Melanie J Bailey
• Journal: Analytical chemistry (Anal Chem) Vol. 95 Issue 39 Pg. 14727-14735 (10 03 2023) ISSN: 1520-6882 [Electronic] United States
• PMID: 37725657 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipids
• Gemcitabine

Topics

• Humans
• Chromatography, Liquid
• Lipidomics (methods)
• Lipids (analysis)
• Tandem Mass Spectrometry
• Pancreatic Neoplasms (drug therapy)
• Gemcitabine
• Pancreatic Neoplasms