[Manganese-based nanoparticles for chemodynamic therapy of gastrointestinal cancer].

Abstract

OBJECTIVE:

To investigate the physicochemical features of glucose oxidase-loaded and manganese-based mesoporous silica nanoparticles (MSN@Mn-GOx) and its antitumor effect against gastrointestinal cancer.

METHODS:

The morphology, particle size and Fenton-like properties of MSN@Mn-GOx nanoparticles were analyzed using transmission electron microscopy (TEM), dynamic light scattering (DLS), Zeta potential analysis, ultraviolet absorption spectroscopy, energy dispersive spectroscopy and X-ray photoelectron spectroscopy. A mouse model bearing human colon cancer HT-29 xenograft was established to examine the antitumor effect of MSN@Mn-GOx using MRI imaging. Reactive oxygen species (ROS) production assay, CCK-8 assay and EdU assay were used to evaluate the in vitro anti-tumor effect of the nanoparticles.

RESULTS:

MSN@Mn-GOx nanoparticles were solid spheres with a diameter of about 100 nm and a Zeta potential of -35 mV. MSN@Mn-GOx had a higher H2O2 catalytic efficiency in glucose containing solution than in glucose-free solution, and showed a stronger Fenton-like properties at pH6.0 than at pH7.4 (P＜0.05). In the tumor-bearing mice, MSN@Mn-GOx treatment dose-dependently enhanced T1 imaging of the tumor (P＜0.01). Compared with the control group and MSN@Mn group, MSN@Mn-GOx induced a significantly higher level of ROS production and a stronger inhibitory effect on the proliferation of gastric and colon cancer cells (P＜0.05).

CONCLUSION:

MSN@Mn-GOx nanoparticles have good chemodynamic properties and a strong anti-tumor effect and provide a potential therapeutic option for gastric cancer.

• Authors: X Lin, M Huang, J Chen, X Zhou, Z Zhong, W Lu, X Huang, T Liu
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 43 Issue 8 Pg. 1432-1439 (Aug 20 2023) ISSN: 1673-4254 [Print] China
• PMID: 37712282 (Publication Type: English Abstract, Journal Article)

Chemical References

• Manganese
• Hydrogen Peroxide
• Reactive Oxygen Species
• Antibodies

Topics

• Humans
• Animals
• Mice
• Manganese
• Hydrogen Peroxide
• Reactive Oxygen Species
• Gastrointestinal Neoplasms (drug therapy)
• Colonic Neoplasms (drug therapy)
• Antibodies