Study on Optimal Extraction and Hypoglycemic Effect of Quercetin.

Abstract

Quercetin was extracted from Portulaca oleracea L. through biphasic acid hydrolysis to investigate its potential as a suppressor of dipeptidyl peptidase IV (DPP-IV) and its hypoglycemic effect in type 2 diabetic mice. The extraction procedure was optimized utilizing the response surface method (RSM) in a single-factor experimental setting. An extraction efficiency of 0.675% was achieved using the following optimized parameters: 0.064 mol/L vitriol, 1 : 109.155 solid-liquid ratio, and 21.408 min ultrasonication. Overall, findings indicate the effectiveness of quercetin extraction. A mouse model for type 2 diabetes was established to receive oral treatment with various quercetin concentrations for 8 weeks. Fasting blood glucose (FBG) and the DPP-IV activity in the serum were significantly reduced. The weight and insulin levels of the mice in the quercetin group were raised compared to those in the model group (P < 0.01). Quercetin dose-dependently inhibited postprandial blood glucose excursions, as demonstrated by the oral glucose tolerance test. These results confirmed that quercetin has hypoglycemic effects and considerably improves insulin sensitivity via DPP-IV targeting.

• Authors: Lei Zhang, Ming-Yue Pan, Tao Li, Zhi-Min Jin, Zhu Liu, Qiu-Yue Liu, Yong Liu, Jia-Yuan Ding, Huan Jiang, Xingchen Hou
• Journal: Evidence-based complementary and alternative medicine : eCAM (Evid Based Complement Alternat Med) Vol. 2023 Pg. 8886503 ( 2023) ISSN: 1741-427X [Print] United States
• PMID: 37674537 (Publication Type: Journal Article)
• Copyright: Copyright © 2023 Lei Zhang et al.