Abstract | BACKGROUND: OBJECTIVES: The purpose of this study was to determine the role of GMP in preventing lipotoxicity-induced myotube atrophy and inflammation. METHODS: C2C12 myoblasts were differentiated to determine the effect of GMP on atrophy and inflammation and to explore its mechanism of action in evaluating various anabolic and catabolic cellular signaling nodes. We also used a lipidomic analysis to evaluate muscle sphingolipid accumulation with the various treatments. Palmitate (0.75 mM) in the presence and absence of GMP (5 μg/mL) was used to induce myotube atrophy and inflammation and cells were collected over a time course of 6-24 h. RESULTS: CONCLUSIONS: In summary, GMP prevented palmitate-induced inflammation and atrophy in C2C12 myotubes. The GMP protective mechanism of action in muscle cells during lipotoxic stress may be related to targeting catabolic signaling associated with cellular stress and proteolysis but not protein synthesis.
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Authors | Naomi Mmp de Hart, Jonathan J Petrocelli, Rebekah J Nicholson, Elena M Yee, Patrick J Ferrara, Eric D Bastian, Loren S Ward, Brent L Petersen, Scott A Summers, Micah J Drummond |
Journal | The Journal of nutrition
(J Nutr)
Vol. 153
Issue 10
Pg. 2915-2928
(10 2023)
ISSN: 1541-6100 [Electronic] United States |
PMID | 37652286
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2023 American Society for Nutrition. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- caseinomacropeptide
- Palmitates
- Peptide Fragments
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Topics |
- Humans
- Whey
(metabolism)
- Palmitates
(toxicity, metabolism)
- Muscle Fibers, Skeletal
(metabolism)
- Muscle, Skeletal
- Muscular Atrophy
(chemically induced, prevention & control)
- Peptide Fragments
- Inflammation
(metabolism)
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